OPTIMALISASI STERILISASI EKSPLAN TUNAS KOPI ROBUSTA (COFFEA CANEPHORA) UNTUK PENANAMAN IN VITRO

POLMANTRI BARASA, NPM 2203100107 (2026) OPTIMALISASI STERILISASI EKSPLAN TUNAS KOPI ROBUSTA (COFFEA CANEPHORA) UNTUK PENANAMAN IN VITRO. Skripsi thesis, Universitas Labuhanbatu.

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Abstract

Optimalisasi Sterilisasi Eksplan Tunas Kopi Robusta (Coffea canephora) untuk Penanaman In Vitro. Dibimbing oleh Kamsia Dorliana Sitanggang dan Badrul Ainy Dalimunthe. Penelitian ini bertujuan mengevaluasi konsentrasi alkohol 40%, 50%, dan 60% pada sterilisasi permukaan eksplan tunas kopi robusta. Penelitian dilaksanakan di Laboratorium Kultur Jaringan Universitas Labuhanbatu pada Mei-Juli 2026. Penanaman dilakukan pada 1 Juli 2026 dan pengamatan berlangsung sampai 8 hari setelah tanam. Penelitian menggunakan Rancangan Acak Lengkap satu faktor dengan tiga taraf perlakuan dan enam ulangan, sehingga terdapat 18 unit percobaan. Alkohol 40% menghasilkan 33,33% eksplan bebas kontaminasi, sedangkan alkohol 50% dan 60% masing-masing menghasilkan 50,00%. Rata-rata waktu muncul kontaminasi berturut-turut adalah 7; 7; dan 8 HST. Kontaminasi didominasi jamur. Analisis ragam terhadap data berkode 100 untuk bebas kontaminasi dan 0 untuk terkontaminasi menghasilkan F-hitung 0,192 dengan p = 0,827. DMRT 5% memberikan notasi yang sama pada semua perlakuan. Uji eksak Fisher-Freeman-Halton juga tidak menunjukkan hubungan yang signifikan antara konsentrasi alkohol dan status kultur (p = 1,000). Secara deskriptif, alkohol 60% menunjukkan kecenderungan paling efektif karena menghasilkan persentase bebas kontaminasi tertinggi bersama alkohol 50% dan waktu muncul kontaminasi paling lambat. Kesimpulan tersebut terbatas pada kondisi penelitian dan periode pengamatan 8 HST. Kata kunci : Alkohol, Coffea Canephora, Eksplan Tunas, Kontaminasi, Kultur Jaringan, Sterilisasi ============================================================================================ Optimization of Shoot Explant Sterilization of Robusta Coffee (Coffea canephora) for In Vitro Establishment. Supervised by Kamsia Dorliana Sitanggang and Badrul Ainy Dalimunthe. This study evaluated 40%, 50%, and 60% ethanol for surface sterilization of robusta coffee shoot explants. The experiment was conducted at the Tissue Culture Laboratory of Universitas Labuhanbatu from May to July 2026. Inoculation was performed on 1 July 2026 and cultures were observed until eight days after planting. A one-factor completely randomized design with three treatments and six replications was used, resulting in 18 experimental units. The percentages of contamination-free explants were 33.33%, 50.00%, and 50.00% for 40%, 50%, and 60% ethanol, respectively. Mean contamination onset was 7, 7, and 8 days after planting. Fungal contamination was dominant. Analysis of variance on data coded as 100 for contamination-free and 0 for contaminated produced F = 0.192 and p = 0.827. The 5% DMRT assigned the same letter to all treatments. The Fisher-Freeman Halton exact test also showed no significant association between ethanol concentration and culture status (p = 1.000). Descriptively, 60% ethanol showed the most favorable tendency because it achieved the joint-highest contamination free percentage and delayed contamination onset. This conclusion is limited to the experimental conditions and the eight-day observation period. Keywords : Alcohol, Coffea Canephora, Contamination, Shoot Explant, Sterilization, Tissue Culture

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Alkohol, Coffea Canephora, Eksplan Tunas, Kontaminasi, Kultur Jaringan, Sterilisasi ================================== Alcohol, Coffea Canephora, Contamination, Shoot Explant, Sterilization, Tissue Culture
Subjects:	S Agriculture > S Agriculture (General) S Agriculture > SB Plant culture
Divisions:	Fakultas Sains Dan Teknologi > Agroteknologi
Depositing User:	Unnamed user with email repository@ulb.ac.id
Date Deposited:	09 Sep 2026 09:40
Last Modified:	09 Sep 2026 09:47
URI:	http://repository.ulb.ac.id/id/eprint/2800

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