OPTIMALISASI STERILISASI EKSPLAN TANAMAN BUNGA MAWAR (Rosa s.p) SECARA IN VITRO

HELFA RAYSA AMELIA, NPM 2203100068 (2026) OPTIMALISASI STERILISASI EKSPLAN TANAMAN BUNGA MAWAR (Rosa s.p) SECARA IN VITRO. Skripsi thesis, Universitas Labuhanbatu.

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Abstract

Mawar (Rosa sp) merupakan salah satu tanaman hias yang memiliki nilai ekonomi dan banyak dimanfaatkan sebagai bunga potong, tanaman pot, maupun tanaman taman. Perbanyakan mawar secara in vitro dapat menjadi salah satu alternatif untuk menghasilkan bahan tanaman secara cepat dan seragam. Namun, keberhasilan kultur jaringan sangat dipengaruhi oleh kondisi eksplan, terutama tingkat kebersihan dan keberhasilan proses sterilisasi. Penelitian ini bertujuan untuk mengetahui efektivitas penggunaan alkohol dengan konsentrasi berbeda terhadap keberhasilan sterilisasi eksplan tanaman bunga mawar secara in vitro. Penelitian dilaksanakan di Laboratorium Kultur Jaringan Tanaman Universitas Labuhanbatu pada bulan Juni–Agustus. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan dua perlakuan, yaitu F1 alkohol 30% dan F2 alkohol 40%, dengan masing-masing sembilan ulangan. Parameter yang diamati meliputi persentase eksplan steril, persentase kontaminasi, waktu muncul kontaminasi, serta persentase kontaminasi bakteri dan jamur. Hasil penelitian menunjukkan bahwa perlakuan F2 menggunakan alkohol 40% menghasilkan persentase eksplan steril sebesar 77,78% dan kontaminasi sebesar 22,22%, sedangkan F1 menggunakan alkohol 30% menghasilkan persentase eksplan steril sebesar 55,56% dan kontaminasi sebesar 44,44%. Kontaminasi pada F1 mulai muncul pada hari ke-3, sedangkan pada F2 mulai muncul pada hari ke-5. Kontaminasi bakteri dan jamur pada F1 masing-masing sebesar 22,22%, sedangkan pada F2 masing-masing sebesar 11,11%. Berdasarkan hasil tersebut, alkohol 40% memberikan hasil sterilisasi yang lebih baik secara deskriptif dibandingkan alkohol 30%, meskipun perbedaannya belum menunjukkan perbedaan nyata secara statistik pada taraf 5%. Kata Kunci: Mawar, Kultur Jaringan, Sterilisasi Eksplan, Kontaminasi ====================================================================================== Rose (Rosa sp ) is one of the ornamental plants with high economic value and is widely used as cut flowers, potted plants, and garden plants. In vitro propagation can be used as an alternative method to produce plant material rapidly and uniformly. However, the success of tissue culture is strongly influenced by the condition of the explants, particularly their cleanliness and the effectiveness of the sterilization process. This study aimed to determine the effectiveness of different alcohol concentrations on the sterilization of rose explants under in vitro conditions. The research was conducted at the Plant Tissue Culture Laboratory of Universitas Labuhanbatu from June to August. The experiment used a Completely Randomized Design (CRD) with two treatments, namely F1: 30% alcohol and F2: 40% alcohol, with nine replications for each treatment. The observed parameters included the percentage of sterile explants, percentage of contamination, time of contamination appearance, and percentages of bacterial and fungal contamination. The results showed that the F2 treatment using 40% alcohol produced a higher percentage of sterile explants, namely 77.78%, with 22.22% contamination, while the F1 treatment using 30% alcohol produced 55.56% sterile explants and 44.44% contamination. Contamination in F1 first appeared on the third day, whereas in F2 it first appeared on the fifth day. Bacterial and fungal contamination in F1 were each 22.22%, while in F2 they were each 11.11%. Based on these results, 40% alcohol provided better sterilization results descriptively than 30% alcohol, although the difference was not statistically significant at the 5% level. Keywords: Rose, Plant Tissue Culture, Eksplan Sterilization, Contamination

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Mawar, Kultur Jaringan, Sterilisasi Eksplan, Kontaminasi===============Rose, Plant Tissue Culture, Eksplan Sterilization, Contamination
Subjects:	Q Science > QK Botany S Agriculture > S Agriculture (General) S Agriculture > SB Plant culture
Divisions:	Fakultas Sains Dan Teknologi > Agroteknologi
Depositing User:	Unnamed user with email repository@ulb.ac.id
Date Deposited:	19 Oct 2026 09:09
Last Modified:	19 Oct 2026 09:09
URI:	http://repository.ulb.ac.id/id/eprint/2927

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